Discrimination and geo-spatial mapping of atmospheric VOC sources using full scan direct mass spectral data collected from a moving vehicle.

Volatile and semi-volatile organic compounds (S/VOCs) are ubiquitous in the environment, come from a wide variety of anthropogenic and biogenic sources, and are important determinants of environmental and human health due to their impacts on air quality. They can be continuously measured by direct mass spectrometry techniques without chromatographic separation by membrane introduction mass spectrometry (MIMS) and proton-transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS). We report the operation of these instruments in a moving vehicle, producing full scan mass spectral data to fingerprint ambient S/VOC mixtures with high temporal and spatial resolution. We describe two field campaigns in which chemometric techniques are applied to the full scan MIMS and PTR-ToF-MS data collected with a mobile mass spectrometry lab. Principal Component Analysis (PCA) has been successfully employed in a supervised analysis to discriminate VOC samples collected near known VOC sources including internal combustion engines, sawmill operations, composting facilities, and pulp mills. A Gaussian mixture model and a density-based spatial clustering of application with noise (DBSCAN) algorithm have been used to identify sample clusters within the full time series dataset collected and we present geospatial maps to visualize the distribution of VOC sources measured by PTR-ToF-MS.

Discrimination of constructed air samples using multivariate analysis of full scan membrane introduction mass spectrometry (MIMS) data.

RATIONALE: Volatile and semi-volatile organic compounds (S/VOCs) are important atmospheric pollutants affecting both human and environmental health. They are directly measured as an unresolved mixture using membrane introduction mass spectrometry (MIMS). We apply chemometric techniques to discriminate, classify, and apportion air samples from a variety of sources. METHODS: Full scan mass spectra of lab-constructed air samples were obtained using a polydimethylsiloxane membrane interface and an electron ionization ion trap mass spectrometer. Normalized full scan spectra were analyzed using principal component analysis (PCA), cluster analysis, and k-nearest neighbours (kNN) for sample discrimination and classification. Multivariate curve resolution (MCR) was used to extract pure component contributions. Similar techniques were applied to VOC mixtures sampled from different woodsmoke emissions and from the headspace above aqueous hydrocarbon solutions. RESULTS: PCA successfully discriminated 32 constructed VOC mixtures from nearly 300 air samples, with cluster analysis showing similar results. Further, kNN classification (k = 1) correctly classified all but one test set sample, and MCR successfully identified the pure compounds used to construct the VOC mixtures. Real-world samples resulting from the combustion of different wood species and those associated with water contaminated with different commercial hydrocarbon products were similarly discriminated by PCA. CONCLUSIONS: Chemometric techniques have been evaluated using full scan MIMS spectra with a series of VOC mixtures of known composition containing known compounds, and successfully applied to samples with known sources, but unknown molecular composition. These techniques have application to source identification and apportionment in real-world environmental samples impacted by atmospheric pollutants.

Implant dentistry in Australia: the present and future. A survey of Australian dentists and specialists.

BACKGROUND: In recent years, implant dentistry has become a routine part of many general dental practices in Australia. However, there has been little information regarding its extent and scope. This investigation aimed to address this issue by surveying Australian general dental practitioners (GDP) and relevant specialists regarding their practice of implant dentistry. METHODS: Anonymous electronic surveys were designed online using SurveyMonkey™ and delivered to the following professional bodies: all seven state/territory branches of the Australian Dental Association, the Australian and New Zealand Academy of Periodontists, the Australian and New Zealand Academy of Oral and Maxillofacial Surgeons, and prosthodontist members of the Australian Prosthodontic Society. The surveys were completed online via SurveyMonkey in 2014. RESULTS: The response rates were 7.61% (N = 801) for GDP, 41.76% (N = 38) for prosthodontists, 34.16% (N = 55) for periodontists and 34.07% (N = 46) for oral and maxillofacial (OMF) surgeons. Among the respondents, 66.37% (N = 521) of GDP, 86.11% (N = 31) of prosthodontists, 82.98% (N = 39) of periodontists and 97.67% (N = 42) of OMF surgeons indicated that they currently practise implant dentistry. A strong perceived need for further education in this field was also expressed by the respondents. CONCLUSIONS: Implant dentistry presently appears to be practised by a significant proportion of GDP across Australia. This is likely to continue to grow in the future.

A comparison of conventional visual and spectrophotometric shade taking by trained and untrained dental students.

BACKGROUND: The aim of this study was to compare the accuracy of shade selection using a spectrophotometer with a conventional method using a shade guide for 'trained' and 'untrained' students. METHODS: The 'trained' group were given a presentation and training exercise on colour science and shade selection and the 'untrained' group were not given any information or training. Each student matched the shade of maxillary right central incisor for eight test subjects using both methods. Differences in colour (∆E) and value (∆L) between the natural teeth and the shade determined by each method were calculated for both trained and untrained students. RESULTS: Overall, the spectrophotometric method was more accurate but 'trained' students tended to be more accurate in matching shade using the visual method and were significantly better in matching the value visually than when using the spectrophotometer. 'Untrained' students matched both the shade and the value more accurately when using the spectrophotometer. CONCLUSIONS: Within the limits of this study, matching the shade of natural teeth using a spectrophotometric device is more accurate than a conventional method using a shade guide. However, knowledge and training on colour science and shade selection significantly affected the results.

A study of osseointegrated dental implants following cremation.

BACKGROUND: The comparison of dental morphology and restorative work for human identification has been well documented. This case study involved documentation of osseointegrated and clinically restored dental implants following cremation. METHODS: The mandible and the maxilla were excised from a head containing implants and cremated. The remains were retrieved, digital and radiographic images were taken and elemental analysis undertaken. The brand of implants was identified utilizing web based search engines. A prosthodontist, known to commonly use this implant system, was approached to ascertain possibilities that matched the data given. RESULTS: Following cremation the implants were identified and a prosthodontist was able to identify the deceased. Two implants in the maxilla had dehiscences on their buccal surfaces, which could not be detected by periapical radiographs. CONCLUSIONS: Dental implants osseointegrated and restored with a prosthetic superstructure were recognizable following severe incineration. It was possible to trace back the identity of the unknown victim to a prosthodontist. Bone dehiscences discovered in this study highlighted how two-dimensional radiographs may not reveal lack of bone support.

The teeth and faces of twins: providing insights into dentofacial development and oral health for practising oral health professionals.

The continuing studies of the teeth and faces of Australian twins and their families in the Craniofacial Biology Research Group in the School of Dentistry at the University of Adelaide began 30 years ago. Three main cohorts of twins have been recruited, enabling various objectives and specific hypotheses to be addressed about the roles of genetic, epigenetic and environmental influences on human dentofacial growth and development, as well as oral health. This paper highlights some key findings arising from these studies, emphasizing those of direct relevance to practising oral health professionals. We also draw on published literature to review the significant developments in relation to the use of precision 2D and 3D imaging equipment, the application of modern molecular techniques, and the development of sophisticated computer software for analysing genetic relationships and comparing complex shapes. Such developments are valuable for current and future work. Apart from the classical or traditional twin model, there are several other twin models that can be used in research to clarify the relative contributions of genetic, epigenetic and environmental contributions to phenotypic variation. The monozygotic (MZ) co-twin model is one particularly valuable method, given that examination of only one pair of MZ twins can provide considerable insights into underlying causes of observed variation. This model can be used in a dental practice environment, with oral health professionals having the opportunity to explore differences in orofacial structures between MZ co-twins who are attending as patients. As researchers have become more aware of the complexities of the interactions between the genome, the epigenome and the environment during development, there is the need to collect more phenotypic data and define new phenotypes that will better characterize variations in growth processes and health status. When coupled with powerful new genetic approaches, including genome-wide association studies and linkage analyses, exciting opportunities are opening up to unravel the causes of problems in craniofacial growth and common oral diseases in human populations.

A laboratory investigation of colour changes in two contemporary resin composites on exposure to spices.

BACKGROUND: The aim of this study was to evaluate colour stability upon exposure to spices of a nano-filled and a micro-hybrid resin composite finished either with Sof-Lex™ discs (SLD) or against plastic strips (PS). METHODS: Forty cylindrical specimens of 3 mm thickness were fabricated from Filtek Supreme XT ™ (FS) and Gradia Direct X™ (GD). The top surface of each specimen was polished with SLD while the bottom surface was finished against PS. All samples were immersed in staining solutions (0.1% weight turmeric, paprika and tamarind) and distilled water at 37 °C. Colour after 0, 24, 72 and 168 hours of immersion was recorded with a reflection spectrophotometer using CIE L*a*b* parameters and the results were statistically analysed with repeated measures of ANOVA and Bonferroni post hoc tests. RESULTS: Among all the staining solutions tested, the highest colour deviation was obtained in the turmeric group. FS finished against PS showed significantly more colour changes compared to specimens polished with SLD, while GD finished against PS were found to be more resistant to colour changes. CONCLUSIONS: Within the limitations of this study all the spices tested have the potential to stain resin composites with turmeric causing the most significant discolouration. Micro-hybrid and nano-filled resin composites appeared to respond differently to staining by spices when either finished with PS or polished with SLD.

The effect of casein phosphopeptide-amorphous calcium phosphate on erosive dentine wear.

BACKGROUND: Erosive tooth wear is a growing concern in clinical dentistry. Our aims were to assess the effect of Tooth Mousse (TM) in managing erosive dentine wear in vitro. METHODS: Opposing enamel and dentine specimens from 36 third molar teeth were worn under a load of 100 N for 75 000 cycles in electromechanical tooth wear machines. In experiment 1, TM was applied continuously at the wear interface and the mean dentine wear rate was compared with those of specimens subjected to continuous application of hydrochloric acid (HCl, pH 3.0) and deionized water (DW, pH 6.1) as lubricants. In experiment 2, specimens were subjected to TM application every 1600 cycles at both pH 3.0 and 6.1, and the mean dentine wear rates were compared with those of specimens worn with continuous application of HCl and DW lubricants. RESULTS: Dentine wear was reduced significantly with continuous application of TM compared with HCl and DW lubricants. Specimens prepared with continuous TM application displayed smooth wear facets, whereas more pronounced microwear details were observed with HCl and DW lubricants. CONCLUSIONS: Both remineralization and lubrication seem to contribute to reduction in dentine wear associated with TM application, although lubrication appears to have a more pronounced effect.

The effect of casein phosphopeptide-amorphous calcium phosphate on erosive enamel and dentine wear by toothbrush abrasion.

OBJECTIVE: In addition to its role as a remineralizing agent in preventing dental caries, calcium product (CPP-ACP) delivered as a mousse (Tooth Mousse, TM) can reduce erosion of enamel and dentine. The aim of this study was to determine whether CPP-ACP could also reduce erosive tooth wear involving toothbrush abrasion. METHODS: Flat, polished enamel and dentine specimens (n=72) were subjected to 10 wear regimes, with each regime involving erosion in 0.3% citric acid (pH 3.2) for 10 min followed by toothbrush abrasion in a slurry of fluoride-free toothpaste and artificial saliva (1:3 ratio by weight) under a load of 2N for 200 cycles. The specimens were immersed in artificial saliva for 2h between wear regimes. In the experimental group 1, TM (containing CPP-ACP) was applied at the beginning of each wear episode for 5 min whereas TM- (without CPP-ACP) was applied in the experimental group 2. No mousse was applied in the control group. RESULTS: TM significantly reduced enamel wear (mean+/-S.E., 1.26+/-0.33 microm in the experimental group 1 vs 3.48+/-0.43 microm in the control group) and dentine wear (2.16+/-0.89 microm in the experimental group 1 vs 10.29+/-1.64 microm in the control group), and dentine wear was significantly less in the experimental group 1 than in the experimental group 2 (5.75+/-0.98 microm). CONCLUSION: The finding that TM reduced erosive tooth wear involving toothbrush abrasion, probably by remineralizing and lubricating eroded tooth surfaces, may have implications in the management of tooth wear.

Retention of cast crown copings cemented to implant abutments.

BACKGROUND: The cementation of crowns to dental implant abutments is an accepted form of crown retention that requires consideration of the properties of available cements within the applied clinical context. Dental luting agents are exposed to a number of stressors that may reduce crown retention in vivo, not the least of which is occlusal loading. This study investigated the influence of compressive cyclic loading on the physical retention of cast crown copings cemented to implant abutments. METHODS: Cast crown copings were cemented to Straumann synOcta titanium implant abutments with three different readily used and available cements. Specimens were placed in a humidifier, thermocycled and subjected to one of four quantities of compressive cyclic loading. The uniaxial tensile force required to remove the cast crown copings was then recorded. RESULTS: The mean retention values for crown copings cemented with Panavia-F cement were statistically significantly greater than both KetacCem and TempBond non-eugenol cements at each compressive cyclic loading quantity. KetacCem and TempBond non-eugenol cements produced relatively low mean retention values that were not statistically significantly different at each quantity of compressive cyclic loading. Compressive cyclic loading had a statistically significant effect on Panavia-F specimens alone, but increased loading quantities produced no further statistically significant difference in mean retention. CONCLUSIONS: Within the limitations of the current in vitro conditions employed in this study, the retention of cast crown copings cemented to Straumann synOcta implant abutments with a resin, glass ionomer and temporary cement was significantly affected by cement type but not compressive cyclic loading. Resin cement is the cement of choice for the definitive non-retrievable cementation of cast crown copings to Straumann synOcta implant abutments out of the three cements tested.

Strong genetic control of emergence of human primary incisors.

Our understanding of tooth eruption in humans remains incomplete. We hypothesized that genetic factors contribute significantly to phenotypic variation in the emergence of primary incisors. We applied model-fitting to data from Australian twins to quantify contributions of genetic and environmental factors to variation in timing of the emergence of human primary incisors. There were no significant differences in incisor emergence times between zygosity groups or sexes. Emergence times of maxillary central incisors and mandibular lateral incisors were less variable than those of maxillary lateral incisors and mandibular central incisors. Maxillary lateral incisors displayed significant directional asymmetry, the left side emerging earlier than the right. Variation in timing of the emergence of the primary incisors was under strong genetic control, with a small but significant contribution from the external environment. Estimates of narrow-sense heritability ranged from 82 to 94% in males and 71 to 96% in females.

Tooth wear prevention: a quantitative and qualitative in vitro study.

BACKGROUND: Management of tooth wear from grinding presents a significant clinical challenge. Acrylic nightguards are often used to protect the teeth, but many patients still grind with these appliances. This study aimed to test the effectiveness of three lubricants in reducing enamel wear by using an electro-mechanical machine under controlled conditions, with a view to undertaking a subsequent longitudinal clinical study. METHODS: Sectioned tooth specimens were worn against each other under different loads and with the addition of three different lubricants: calcium fluoride (CaF) powder, olive-oil, and a combination of calcium fluoride with olive-oil in the form of a slurry. Wear rates of enamel only were quantified by weighing the specimens, and resin replicas of the worn tooth surfaces were made for examination under a scanning electron microscope. RESULTS: All three lubricants reduced the amount of enamel wear significantly compared with wearing specimens without adding lubricants. Wear rate was influenced by the type of lubricant and the load applied. Wear rates were significantly less for olive-oil and the olive-oil/CaF slurry compared with CaF alone. The microwear detail differed between the three lubricants. CONCLUSIONS: This study has shown that enamel wear can be reduced using dry or wet lubricants between opposing teeth that are worn under controlled conditions. Further research is required to clarify their possible clinical applications.

A model for the prediction of tooth wear in individuals.

BACKGROUND: Easily applied, age-specific standards are useful in assisting clinicians to decide whether the extent of tooth wear observed in a given patient at a specific age is acceptable or whether intervention is indicated. METHOD: A simple method of scoring tooth wear and previously established mathematical relationships between tooth wear scores and age, provide the basis of a method for predicting tooth wear scores. In its most specific form, the method can be used to predict tooth wear scores for individual patients at subsequent ages. Alternatively, tables or graphs can be consulted to provide a less precise prediction of tooth wear that can assist clinicians to decide whether the amount of wear reflects high, moderate or low rates of wear at the specified age. CONCLUSION: In cases where there is no reason to believe that aetiological factors have changed significantly over time, data obtained from the methods described can assist clinicians to plan appropriate management for patients presenting with unacceptable levels of tooth wear.

Anxiety-related Stroop interference in adolescents.

A group of 16- to 18-year-old students was presented with threat-related and neutral Stroop stimuli on separate cards. Participants were assigned to anxiety groups on the basis of their scores on the Beck Anxiety Inventory (BAI; A. T. Beck & R. A. Steer, 1990). It was found, as predicted, that the high-anxiety group took significantly longer to identify the color of the threat-related word than the neutral words, whereas there was no difference for the low-anxiety group. There was a significant linear relationship between interference on the task and BAI scores, showing that as anxiety increases there is a corresponding increase in interference produced by the threat-related stimuli when compared with the neutral stimuli. This study demonstrates an anxiety-related Stroop interference effect for adolescents consistent with that reported in the adult literature.

Experimental studies of human dentine wear.

Previous in-vitro studies have described the relation between rates of enamel wear and variables such as applied load and lubricant pH. The aim here was to extend understanding of tooth-wear processes by considering the rate of wear in human dentine. Enamel was removed from extracted third molar teeth that had been sectioned mesiodistally. Moisture fluctuation within dentine was minimized by conducting all procedures under copious irrigation or in sealed containers of lubricant at pH 7.0. Specimens were subjected to wear using a purpose-built apparatus at loads of 6.2, 9.95 and 13.2 kg. All experiments were done with a unidirectional wear stroke of 3 mm at a rate of 80 cycles/min for 75 min and repeated for 75 min. Dentine wear was assessed by specimen weight loss. At pH 7.0, wear rates ranged from 0.50 mg/10(3) cycles at a load of 6.2 kg to 0.77 mg/10(3) cycles when a load of 13.2 kg was applied. At higher loads, dentine wear rates were similar to those of enamel. Increasing load is thus associated with a progressive increase in the rate of dentine wear. This relation differs significantly from that for enamel, reflecting fundamental differences in the composition and structure of these tissues.

Wear of human enamel: a quantitative in vitro assessment.

Many factors influence the extent and rate at which enamel wears. Clinical studies in humans are limited by difficulties in the accurate quantification of intra-oral wear and by a lack of control over the oral environment. The purpose of this study was to determine the wear characteristics of human dental enamel under controlled experimental conditions. An electro-mechanical tooth wear machine, in which opposing enamel surfaces of sectioned, extracted teeth were worn under various conditions, was used to simulate tooth grinding or bruxism. Enamel surface wear was quantified by weight to an accuracy of 0.1 mg, with water uptake and loss controlled. The variables considered included the structure and hardness of enamel, facet area, duration of tooth contact, relative speed of opposing surfaces, temperature, load, pH, and the nature of the lubricant. Enamel wear under non-lubricated conditions increased with increasing load over the range of 1.7 to 16.2 kg. The addition of a liquid lubricant (pH = 7) reduced enamel wear up to 6.7 kg, but when the load increased above this threshold, the rate of wear increased dramatically. With the viscosity of the lubricant constant and pH = 3, the rate of wear was further reduced to less than 10% of the non-lubricated rate at 9.95 kg, after which the rate again increased substantially. Under more extreme conditions (pH = 1.2, simulating gastric acids), the wear was excessive under all experimental loads. When saliva was used as a lubricant, the amount of wear was relatively low at 9.95 kg, but rapid wear occurred at 14.2 kg and above. These results indicate that under non-lubricated conditions, enamel wear remains low at high loads due to the dry-lubricating capabilities of fine enamel powder. Under lubricated conditions, low loads with an acidic lubricant lead to little enamel wear, whereas very low pH results in a high rate of wear under all loads.

Cephalometric analysis of masseter muscle and dentoskeletal morphology in dentate and edentulous humans.

A study was done to clarify the attachment position of the superficial masseter muscle and its relationship with craniofacial morphology in dentate and edentulous subjects. Data were obtained from lateral cephalometric radiographs of a total of 39 cadavers in which the superficial masseter muscle had been defined with colloidal barium. Principal component analysis provided evidence that the lower masseter muscle width was associated with the size of the ramus, and related to the position of the anterior margin of the muscle. Linear discriminant function data suggested that ramus width, coronoid height and the distance between the anterior margin of the masseter muscle and the mandibular notch contributed most to the observed difference between dentate and edentulous subjects. The derived function correctly assigned 100% of dentate and 95% of edentulous subjects. The results of this study indicated that a reduction of masticatory function was associated with the position of the anterior border of the masseter muscle insertion and also with differences in ramus dimension, the most significant of which were differences in the coronoid process and gonial angle. In general, age was not a significant determinant of variation in superficial masseter muscle dimensions or orientation.

Dental arch form in three Pacific populations: a comparison with Japanese and Australian aboriginal samples.

The purpose of this study was to provide information about arch shape variations among South Pacific populations. The application of Fourier transforms, Y(phi i) = ao/2 + sigma (aicos phi i + bisin phi i) was used to separate two components of variation; size and shape. Dental arch size can be described by one coefficient (ao), while the shape can be summarized by the first three harmonics (amp1-3) in the Fourier series. The materials used in this study were dental casts of South Pacific populations (Fiji, Western Samoa and Kiribati), Australian Aboriginals and Japanese. Fijians, Western Samoans and Kiribati people have larger upper and lower dental arches than that of Japanese. These populations were separated by the arch size and first Fourier amplitudes which showed the arch depth/width ratio. The Fijian upper and lower dental arches were significantly larger than those found in other populations and was characterized by a wide posterior arch breadth. The Western Samoan and Kiribati arch shape was more similar to the Japanese arch shape than the Fijian. Distribution patterns of arch shape characteristics in these populations showed the same tendencies in the upper and lower arches.

Removable prosthodontics: a survey of practices and attitudes among South Australian dentists.

All dentists registered in South Australia were surveyed in July 1993 in relation to removable prosthodontics. The replies indicated that removable prosthodontics constituted a significant part of most dental practices. Most dentists felt that removable prosthodontics should be an essential part of the undergraduate curriculum. They felt that more expertise is now required to manage the treatment of the ageing population with either their first or replacement removable prostheses. The majority of dentists felt that their own undergraduate training had equipped them well to manage removable prosthodontic treatment for their patients; however, many helpful suggestions were made on areas that they thought required more emphasis.

Genetic and environmental influences on facial profile.

Observed variation in facial morphology results from the influences of both genetic and environmental factors. To investigate the role of genetic influences on variation in facial profile, lateral photographs of 23 male and 19 female monozygous (MZ), and 15 male and 22 female dizygous (DZ) twin pairs were selected from the records of the South Australian Twin Study. Soft tissue landmarks and facial profiles were identified, traced and digitized. Linear and angular measures defined by the landmarks were calculated and a series of Fourier functions derived to describe the facial profiles. These Fourier amplitudes were interpreted in terms of the specific regions which they represented. Correlations within MZ pairs were consistently higher than within DZ pairs for both the facial dimensions and the Fourier amplitudes, providing evidence of significant genetic contribution to facial convexity, facial height and facial depth. Variability in nose and lip morphology appeared to be under stronger environmental influence.